Raised Serum Homocysteine Level is Associated with Coronary Artery Disease in Young Patients

Rokshana Begum¹*, Mohammad Monzurul Alam Bhuiyan², Sheuly Ferdoushi³, Khandoker Abdur Rahim⁴, Dipal K. Adhikari⁵, Shihab Md. Rezoyanur Rahman⁶, Amit Kumar Pramanik⁷, AKM Shahidur Rahman⁸, Farzana Islam Khan⁹, Mukul Chandra Nath¹⁰, Md. Saiful Islam³

¹Consultant, Department of Laboratory Medicine, Bangabandhu Sheikh Mujib Medical University (BSMMU), Dhaka, Bangladesh

²Assistant professor, Department of Laboratory Medicine, Bangabandhu Sheikh Mujib Medical University (BSMMU), Dhaka, Bangladesh

 ³Associate professor, Department of Laboratory Medicine, Bangabandhu Sheikh Mujib Medical University (BSMMU), Dhaka, Bangladesh

⁴Consultant, Department of Orthopaedic surgery, Bangabandhu Sheikh Mujib Medical University (BSMMU), Dhaka, Bangladesh

⁵Associate professor, Department of Cardiology, Bangabandhu Sheikh Mujib Medical University (BSMMU), Dhaka, Bangladesh

⁶Pathologist, Department of Pathology and Blood Transfusion, Gaibandha General Hospital, Gaibandha, Bangladesh

⁷Medical officer, Department of Transfusion Medicine, Rajshahi Medical College Hospital, Rajshahi, Bangladesh

⁸Medical officer, Department of Nephrology, Bangabandhu Sheikh Mujib Medical University (BSMMU), Dhaka, Bangladesh

⁹Associate professor and Head of the Department, Department of Community Medicine, Ashiyan Medical College, Dhaka, Bangladesh

¹⁰Assistant Professor and Head of the Department, Department of Psychiatry, Marine City Medical College, Chattogram, Bangladesh

*Corresponding author: Dr. Rokshana Begum, Consultant, Department of Laboratory Medicine, Bangabandhu Sheikh Mujib Medical University (BSMMU), Dhaka, Bangladesh

Received: 13 June 2022; Accepted: 21 June 2022; Published: 27 June 2022

1. Introduction

Coronary artery disease (CAD) is a disease in which there is a narrowing or blockage of the coronary arteries that usually caused by atherosclerosis [1]. Coronary artery disease (CAD) is defined as any patient having myocardial infarction (MI) and ischemia evidenced by electrocardiogram (ECG), echocardiogram (Echo) and coronary angiography (CAG) [1]. Coronary artery disease (CAD) is one of the leading causes of death worldwide [2]. Coronary artery disease is more diffuse and aggressive in adult population, although it strikes at a younger age [3, 4]. In South Asians, the prevalence of coronary artery disease is rising, as is the burden of cardiovascular disease [5]. It affects people in South Asia earlier than it does those in Europe and Western countries [5]. Acute myocardial infarction (AMI) is the primary cause of death in persons over the age of 40, and even in those under the age of 40 [6]. It was reported that, the risk of acute myocardial infarction is higher in the younger population than adults [7]. Several risk factors are linked to coronary artery disease [8, 9]. Some are modifiable like-hypertension, dyslipidemia, smoking, diabetes mellitus, obesity, lack of physical activity, an unhealthy diet habit and excessive stress; while others (genetic, racial) are not [8, 9]. Beyond established risk factors, many conditions are gaining attention as emergent risk factors for coronary artery disease [2, 10]. A higher level of homocysteine (Hcy) in the blood is regarded as a new risk factor and a treatable condition of CAD [2, 10. 11]. Homocysteine (Hcy) is a sulfur-containing non-protein forming amino acid, which occurs naturally in the blood plasma [12]. It is an intermediate product in the normal biosynthesis of methionine and cysteine [12]. It is produced via demethylation of dietary methionine, which is abundant in animal protein [12]. Normal homocysteine concentrations in healthy subjects range from 5 µmol/L to 15 µmol/L [13]. Hyperhomocysteinemia (>15 µmol/L) causes endothelial cell damage, smooth muscle cell hyperplasia and vascular occlusion, all of which contribute to atherosclerosis [14]. It was reported that, about half of patients with untreated hyperhomocysteinemia develop different cardiovascular disease before the age of 30 [12, 15]. Moreover, hyperhomocysteinemia is associated with an increased risk of athero-thrombotic disease [12, 14-16]. Atherosclerosis leads to coronary artery disease like- myocardial infarction/ischemia [17]. Recent studies suggested that, nearly one-third of patients with atherosclerosis had elevated plasma homocysteine [15-17]. Elevation by 12% above the upper limit of normal plasma homocysteine (15 µmol/L) is associated with a three-fold increase in the risk of acute myocardial infarction (AMI) [15]. Therefore a high homocysteine level considered as a risk factor for coronary artery disease (CAD) [15-18]. Though age is the most important risk factor for coronary artery disease (CAD), it is becoming more common in young adults [3, 11]. Several studies suggested that hyperhomocysteinemia is associated with coronary artery disease in young patients [2, 10, 11]. Early detection of this risk factor may improve prognosis and helps to prevent coronary artery disease particularly among young patients. In this background this study aimed to evaluate serum homocysteine levels in young and adult patients with coronary artery disease.

2. Methodology

2.1 Study design

This cross-sectional study was conducted in the Department of Laboratory Medicine, Bangabandhu Sheikh Mujib Medical University (BSMMU), Dhaka, Bangladesh from March 2021 to February 2022. The study was approved by the Ethical Review Committee, BSMMU, Dhaka, Bangladesh.

2.2 Study population

Diagnosed cases of young and adult coronary artery disease patients admitted in inpatient department (IPD) of Department of Cardiology, BSMMU were enrolled into the study. Patients with coronary artery disease were confirmed by electrocardiography (ECG), echocardiography (ECHO) and coronary angiography (CAG). A total of 52 patients [26 young patients (Group I) and 26 adult patients (Group II)] were selected purposively according to the selection criteria. Young [age<40 years (18-39 years)] and adult patients (age≥40 years) of both sexes with coronary artery disease (CAD) were included. Patients with renal/hepatic failure, patients having any types of malignancy, patients with myocarditis or infective endocarditis and patients on vitamin B₁₂ or folate supplementation were excluded from the study.

2.3 Recruitment of study subjects

From inpatient department of Department of Cardiology, BSMMU; diagnosed cases of coronary artery disease (CAD) patients were included in this study. Coronary artery disease (CAD) cases were diagnosed by cardiologist according to electrocardiography (ECG), echocardiography (ECHO) and coronary angiography (CAG). Report of ECG, ECHO and CAG were collected from patient files. Report of serum lipid profile [Total Cholesterol (TC), High Density Lipoprotein- Cholesterol (HDL- C), Low Density Lipoprotein- Cholesterol (LDL- C) and Triglyceride (TG)] was also collected from patient files.

2.4 Study procedure

After ethical clearance from institutional review board (IRB), diagnosed case of young and adult coronary artery disease (CAD) patients who admitted in Department of Cardiology, BSMMU were selected. Informed written consent was taken from each study participant prior to the enrollment. Patients ECG, ECHO and CAG reports were evaluated to diagnose as coronary artery disease. Their demographic data, detailed history and relevant clinical examination findings were recorded.  Risk factor profiles including- smoking, hypertension, family history of coronary artery disease, diabetes mellitus, dyslipidaemia and obesity were noted. Then fasting venous blood of the study subjects was collected for estimation of serum homocysteine level.

2.5 Collection, processing and analysis of blood samples

With all aseptic precaution 2 ml venous blood (fasting) from the median cubital vein of each study subject was collected in a red test tube following standard procedure. After collection, tubes were labeled with the patient’s identification number and kept in vertical position for 30 minutes. Then blood was centrifuged at 3000 rpm/minutes for 5 minutes in room temperature (22°C - 24°C). Serum was separated by micro-pipette and collected into appendrope, then preserved at −20°C temperature until further analysis. Collected every test sample was run two successive days per week. Serum homocysteine level was estimated by automated immunoassay analyzer with the principle of chemiluminescent microparticle immunoassay (CMIA) in the Department of Biochemistry and Molecular Biology, BSMMU, Dhaka, Bangladesh.

2.6 The reference value of serum homocysteine

The reference normal range of serum homocysteine (5-15 µmol/L) used in this study was according to the Biochemistry and Molecular Biology Laboratory, Department of Biochemistry and Molecular Biology, Bangabandhu Sheikh Mujib Medical University(BSMMU) Dhaka, Bangladesh [13]. Elevated serum homocysteine is referred to as hyperhomocysteinemia (HHCy). Hyperhomocysteinemia has been classified by Kang et al. as follows [19] -

1. Moderate hyperhomocysteinemia (16 to 30 µmol/L)
2. Intermediate hyperhomocysteinemia (31 to 100 µmol/L)
3. Severe Hyperhomocysteinemia (>100 µmol/L)

2.7. Statistical analysis

Data were processed and analyzed using Statistical Package for Social Sciences (SPSS) version 26. Quantitative variables were measured as mean ± SD (standard deviation). Qualitative variables were measured as the frequency and percentage. Chi-square (x²) test and unpaired t-test was performed to analyze the data. A p value <0.05 was considered as statistically significant.

3. Results

This cross-sectional study was intended to assess serum homocysteine level in young and adult patients with coronary artery disease. A total of fifty two (52) patients with coronary artery disease were included. Of them; 26 young patients (age <40 years) were categorized as group I and 26 adult patients (age ≥40 years) were categorized as group II. Among the young patients (group I); 20 (76.9%) were male and 6 (23.1%) were female.  While in the group II (adult patients); 22 (84.6%) were male and rest 4 (15.4%) were female (Figure- 1).

Figure- 1: Gender distribution of the patients in two groups

On risk factor analysis among the study subjects, it was observed that smoking habit was significantly higher in group I than group II patients (65.4% versus 34.6%, p= 0.027). Diabetes mellitus was significantly higher in group II than group I (61.5% versus 26.9%,p= 0.012). Hypertension and dyslipidemia were comparatively higher in group II than group I (76.9% versus 61.5%, p= 0.229 and 53.8% versus 46.2%, p = 0.579 respectively). Family history of CAD and obesity were relatively more in group I than group II (53.8% versus 46.2%,p = 0.579 and 53.8% versus 42.3%, p = 0.405) (Table- I).

Table I: Distribution of risk factors in two groups among the study subjects (N=52)

*Multiple response, Chi-square test was performed to compare the differences between two groups, s=significant, ns= not significant

Table II shows the comparison of serum lipid profile [Total Cholesterol (TC), High Density Lipoprotein- Cholesterol (HDL- C), Low Density Lipoprotein- Cholesterol (LDL- C) and Triglyceride (TG)] findings among the study subjects. The mean (±SD) serum total cholesterol (219.65±40.50 mg/dl versus 182.85±42.17 mg/dl, p = 0.002), HDL- C (52.81±11.03 mg/dl versus 46.31±7.07mg/dl, p = 0.015) and triglyceride (283.69±69.99 mg/dl versus 223.42±52.08 mg/dl, p =0.001) levels were significantly higher in group II patients than group I patients. While no significant difference was observed in mean (±SD) LDL- C level among the groups (128.04±41.99mg/dl versus 128.5 ± 33.12mg/dl, p= 0.965) (Table- II).

Table II: Comparison of lipid profile between the groups (N=52)

Unpaired t-test was performed to compare differences between two groups, s= significant, ns= not significant

Table III shows the electrocardiographic (ECG) findings in two groups. We found that myocardial infarction (MI) was significantly prevalent in group I patients than group II patients (65.4% versus 34.6%, p = 0.027). On the other hand ischemia was significantly high in group II patients than group I patients (65.4% versus 34.6%, p = 0.027) (Table- III).

Table III: Electrocardiographic (ECG) findings of the study subjects (N=52)

Chi-square test was performed to compare the differences between two groups, s= significant

Table- IV shows the echocardiographic findings among the study patients in two groups. Presence of echocardiographic regional wall motion abnormalities (RWMA) was more frequent in group I patients than group II patients (57.7% versus 38.5%, p=0.165) (Table- IV).

Table- IV: Echocardiographic (ECHO) findings of the study subjects (N=52)

Chi-square test was performed to compare the differences between two groups, ns= not significant

Table- V shows the angiographic findings in two groups. Single vessel disease was significantly more prevalent in group I patients than group II patients (61.5% versus 23.1%, p= 0.005). While, double vessel disease (50.0% versus 26.9%, p = 0.087) and triple vessel disease (26.9% versus 11.5%, p= 0.159) were comparatively more frequent in group II patients than group I patients (Table- V).

Table- V: Angiographic findings of the study subjects (N=52)

Chi-square test was performed to compare the differences between two groups, s= significant, ns= not significant

Among the study population the mean (±SD) serum homocysteine level was significantly high in group I patients than that in group II patients (21.93±7.56 µmol/L and 12.04±5.33 µmol/L, p=0.001) (Table- VI).

Table- VI: Comparison of serum homocysteine level between two groups (N=52)

Unpaired t-test was performed to compare the difference between two groups, s= significant

Data analysis revealed that, serum homocysteine level was within normal limit among 22 (84.6%) patients in group II. Patients with moderate hyperhomocysteinemia and intermediate hyperhomocysteinemia was significantly present in group I than group II [18 (69.2%) patients versus 4 (15.4%) patients and 8 (30.8%) patients versus 0 (0.0%) patients, p <0.001]. Patient with severe hyperhomocysteinemia was not observed in this study (Table- VII).

Table- VII: Severity of serum homocysteine levels in two groups (N=52)

Chi-square test was performed to compare the differences between two groups, s= significant

4. Discussion

Coronary artery disease (CAD) is one of the major public health problems. It is the leading cause of morbidity and mortality in both developed and developing countries [20]. The incidence is doubled in South Asia than in any other regions of the world [5, 21]. CAD is the leading cause of death in people aged more than 40 years and even in the younger individuals [20]. Coronary artery disease is a serious condition and early diagnosis is necessary to halt the disease progress. High level of homocysteine causes- proliferation/thickening of vascular smooth muscle cells, oxidative stress and endothelial dysfunction which can lead to coronary artery disease [22]. During recent years it has been suggested that raised serum homocysteine level is significantly associated with the coronary artery disease [20-21]. Therefore the aim of this study was to evaluate the serum homocysteine level in young and adult patients with coronary artery disease. Total 52 patients with coronary artery disease were included. Of them 26 were young patients [(age <40), group I] and 26 were adult patients [age (≥40 years), group II]. Among the young patients; 20 were male and 6 were female. While among the adult patients; 22 were male and 4 were female. Male patients were comparatively predominant in both groups. This finding was consistent with similar previous studies [10, 21, 23].

The current study demonstrates that baseline risk factors had an important role in identifying young and adult patients at risk for CAD. Traditional risk factors such as smoking, hypertension, diabetes mellitus, dyslipidemia, family history of CAD and obesity were more prevalent in CAD [10, 23]. Among the study patients, smoking habit was found significantly higher in young than adults (64.4% versus 34.6%, p= 0.027). In accordance Islam et al. found a significant high prevalence of smoking in young than adults [23]. In this present study, diabetes mellitus was found significantly higher in adults than young patients (61.5% versus 26.9%, p= 0.012). Similar finding was observed in a couple of previous studies [10, 23]. In this study, other risk factors like hypertension, dyslipidemia were relatively higher in adults than young patients (76.9% versus 61.5% and 53.8% versus 46.2% respectively). While family history of CAD and obesity were more frequent in young patients than adults (53.8% versus 46.2% and 53.8% versus 42.3% respectively).These findings were closely resembled with related previous studies [14, 23, 24].

In this present study, serum fasting lipid profile of both young and adult groups was evaluated. The mean (±SD) serum total cholesterol (219.65±40.50 mg/dl versus 182.85±42.17 mg/dl, p = 0.002), HDL- C (52.81±11.03 mg/dl versus 46.31±7.07 mg/dl, p = 0.015) and triglyceride (283.69±69.99 mg/dl versus 223.42±52.08 mg/dl, p =0.001) levels were significantly higher in group II patients than group I patients. But no significant difference was observed in mean (±SD) LDL- C level among the groups (128.04±41.99 mg/dl versus 128.5 ± 33.12 mg/dl, p= 0.965).In this context Ibrahim et al. also found that mean cholesterol was significantly higher in young than adult group (231.0 versus. 217.8, p= 0.05) [25]. While opposite result was observed in another previous study [26].They found no significant differences in serum LDL, HDL and serum triglyceride levels among the groups [26]. This variability of lipid profile may be due to difference in genetic makeup, lifestyle and dietary/nutritional habits among different population.

In this study, it was observed that myocardial infarction (MI) was more significantly associated with young patients than adults (65.4% versus 34.6%, p=0.027). This finding was in a line of similar previous studies [27, 28]. The present study demonstrated that regional wall motion abnormality (RWMA) was found more frequent in young than adults (57.7% versus 38.5%, p= 0.165). In a previous study it also found that RWMA was relatively higher among young patients than adults [44.4% versus 33.3%,p=0.4858] [28]. In this present study, single vessel disease was found significantly higher in young than adults (61.5% versus 23.1%, p= 0.005). In accordance Bush et al. reported that single vessel disease was significantly higher in young than adults (39.6% versus 27.1%, p=0.001) [5]. This current study also showed that the prevalence of double vessel disease (50.0% versus 26.9%, p=0.087) and triple vessel disease (26.9% versus 11.5%, p=0.159) were comparatively high in adults than young patients. This finding was consistent with a related previous study [5].

In this current study, we found that mean serum homocysteine level was significantly higher in young group than adult group (21.93±7.56 µmol/L versus 12.04±5.33 µmol/L, p=0.001). This finding was supported by a couple of previous studies [15-17]. In this present study, it was observed that severity of serum homocysteine level was significantly associated with young patients than adults. Serum homocysteine level was found within normal limit among 84.6% patients in adult group. On the other hand, patients with moderate hyperhomocysteinemia and intermediate hyperhomocysteinemia were significantly frequent in young patients group [69.2% versus 15.4% and 30.8% versus 0.0%, p <0.001]. Patient with severe hyperhomocysteinemia was not observed in this study. These results were consistent with a similar previous study [15]. This study observed that young patients with coronary artery disease had a raised serum homocysteine level than adults. Therefore, present study finding may help in disease evaluation of early coronary artery disease in young individuals.

5. Conclusion

This study concluded that, serum homocysteine level was significantly higher in young patients with coronary artery disease than adult patients with coronary artery disease. Therefore, high level of serum homocysteine may be used for detection and disease evaluation for coronary artery disease in young patients. Further studies warranted to elucidate the fact.

Limitations of the study

It was a single center study with a relatively small size.

Recommendations

Multicenter broad based prospective cohort study is required to confirm the findings of this present study.

Conflict of interest

The authors declare no conflicts of interest regarding the publication of this paper.

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